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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 38-72 amino acids from the N-terminal region of human GNL3. Antigen Region: 38-72 aa. |
| Target | GNL3 |
| Molecular Weight | 61993 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
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GNL3 Antibody (N-term) [orb1788065]
IF, WB
Human
Rabbit
Polyclonal
Unconjugated
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Fluorescent image of Hela cells stained with GNL3 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). DAPI was used to stain the cell nuclear (blue).
All lanes: Anti-GNL3 Antibody (N-term) at 1:1000 dilution. Lane 1: F9 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
GNL3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
GNL3 Antibody (N-term) (orb1788084)
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