Aconitase 2 (aa541-555) Antibody

SKU: orb20461

Description

Goat polyclonal antibody to ACO2

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:16000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
Reactivity	Human, Mouse, Porcine, Rat
Predicted Reactivity	Bovine, Canine
Application Notes	IHC: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5μg/ml.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Aconitase 2 (aa541-555)
Protein Sequence	QDTYQHPPKDSSGQH
Molecular Weight	85.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti aconitase 2, mitochondrial antibody, anti ACONM antibody, anti MGC20605 antibody, anti MGC33908 antibody, anti ACO2 antibody

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Western blot analysis of heart lysate (35µg protein in RIPA buffer) using Aconitase 2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human breast tissue using Aconitase 2 antibody

Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Adipose lysates using ACO2 antibody.

Western blot analysis of staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates using ACO2 antibody.

Quick Database Links

Gene Symbol

Aconitase 2 (aa541-555)

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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