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Goat anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody

SKU: orb19517

Description

Goat polyclonal antibody to Interferon gamma

Research Area

Musculoskeletal & Connective Tissue Research; Cell Biology

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA: 1:16000, IHC-P: 10 μg/ml
ReactivityHuman
Application Notes
ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml.WB: Approx 16kDa band observed in Human Lymph Node and Spleen lysates (calculated MW of 16.7kDa according to NP_001614.3. Recommended concentration: 1-3µg/ml.

Key Properties

ClonalityPolyclonal
TargetAIF1 / IBA1 (isoforms 1 + 3)
Protein SequenceYEEKAREKEKP
Molecular Weight10.5; 16.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
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FC
Flow Cytometry
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IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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