Goat Anti-AIF1/IBA1 isoform 1 and 3 (Rat reactivity only) Antibody

SKU: orb1994919

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to Rat NP_058892.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Reactivity	Rat

Key Properties

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Host	Goat
Immunogen	Peptide with sequence C-TGPPAKKAISELP.,, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Disclaimer	For research use only

Alternative Names

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AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1

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Goat Anti-AIF1/IBA1 isoform 1 and 3 (Rat reactivity only) Antibody

1.5 µg/mL staining of Rat Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

RefSeq (Protein):NP_001614.3, NP_116573.1

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001614.3, NP_116573.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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