Goat anti-AMACR (aa312-326) Antibody

SKU: orb195339

Description

Goat polyclonal antibody to AMACR

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	WB: Approx 40-42kDa band observed in Human Kidney, Liver and Coloretal cancer lysates, and in lysates of cell line CaCo-2, and approx 40.45kDa band observed in lysates of cell line LNCaP (calculated MW of 42.4kDa according to NP_055139.4) and 43.9kDa accordi. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	AMACR (aa312-326)
Protein Sequence	RGSFITSEEQDVSPR
Molecular Weight	42.4; 22.2; 43.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti AMACR antibody, anti alpha-methylacyl-CoA racemase antibody, anti AMACRD antibody, anti CBAS4 antibody, anti RACE antibody, anti RM antibody, anti 2-methylacyl-CoA racemase antibody

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Immunohistochemical staining of Human Kidney using AMACR antibody

Western blot analysis of CaCo-2 (A) and LNCaP (B) cell lysate using AMACR antibody

Western blot analysis of Human Kidney (A), Liver (B) and Colorectal cancer (C) lysate using AMACR antibody

Quick Database Links

Gene Symbol

AMACR (aa312-326)

NCBI Gene ID

23600

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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