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Aminopeptidase A Antibody

SKU: orb19052

Description

Goat polyclonal to Aminopeptidase A

Research Area

Epigenetics, Stem Cells

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 3-5 μg/ml
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Mouse, Porcine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration, 3-5ug/ml.WB: Approx 170kDa band observed in human brain and testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3 μg/ml.

Key Properties

ClonalityPolyclonal
TargetAminopeptidase A
Protein SequenceEQYQKTSLAQEKEK
Molecular Weight109
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti aminopeptidase A antibody, anti ENPEP antibody, anti glutamyl aminopeptidase (aminopeptidase A) antibody, anti HGNC:3355 antibody, anti APA antibody, anti gp160 antibody, anti CD249 antibody, anti glutamyl aminopeptidase antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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