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Goat anti-Annexin A11 Antibody
SKU: orb18454
Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | Annexin A11 |
| Protein Sequence | YPGYPPPPGGYPP |
| Molecular Weight | 54.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti ANXA11 antibody, anti annexin A11 antibody, anti ANX11 antibody, anti CAP50 antibody, anti annexin XI antibody, anti autoantigen, 56-kD antibody, anti calcyclin-associated annexin 50 antibody, anti RP11-369J21 antibody, anti OTTHUMP00000019957 antibody, anti OTTHUMP00000019958 antibody, anti antibody
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orb18454 staining (0.2ug/ml) of HeLa lysate (RIPA buffer, 30ug total proteinper lane). Primary incubated for 1 hour. Detected by western blot usingchemiluminescence.
orb18454 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb18454 (0.01µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-Annexin A11 Antibody (orb18454)
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