Goat anti-Bin3 Antibody

SKU: orb20001

Description

Goat polyclonal to Bin3

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:16000, WB: 0.3-1 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 75kDa band observed in lysates of cell line K562 (calculated MW of 74.4kDa according to NP_062552.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Bin3
Protein Sequence	TPHNTSKGFQRP
Molecular Weight	74.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti MEPCE antibody, anti methylphosphate capping enzyme antibody, anti BCDIN3 antibody, anti FLJ20257 antibody, anti bin3, bicoid-interacting 3 antibody, anti bin3, bicoid-interacting 3, homolog antibody

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orb20001 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Bin3

NCBI Gene ID

56257

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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