BMI1 (aa237-251) Antibody

SKU: orb233612

Description

Goat polyclonal antibody to BMI1

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human, Mouse, Porcine
Predicted Reactivity	Canine
Application Notes	ELISA: antibody detection limit dilution 1:128000.WB: Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20(15):2110-20. PMID: 16882984). Recommended concentration: 0.1-0.3µg/ml. Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1 ). Recommended concentration: 1-3µg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	BMI1 (aa237-251)
Molecular Weight	36.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti BMI1 antibody, anti BMI1 polycomb ring finger oncogene antibody, anti FLVI2/BMI1 antibody, anti PCGF4 antibody, anti RNF51 antibody, anti B lymphoma Mo-MLV insertion region 1 homolog antibody, anti flvi-2/bmi-1 antibody, anti murine leukemia viral (bmi-1) oncogene homolog antibody, anti polycomb complex protein BMI-1 antibody, anti polycomb group RING finger protein 4 antibody, anti polycomb antibody

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Western blot analysis of K562 nuclear lysate using BMI1 antibody

Western blot analysis of Pig Bone Marrow lysate using BMI1 antibody

Western blot analysis of NIH3T3 nuclear lysate using BMI1 antibody

Immunohistochemical staining of Human Testis using BM1 antibody.

Quick Database Links

Gene Symbol

BMI1 (aa237-251)

NCBI Gene ID

648

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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