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Calnexin Antibody

SKU: orb20292

Description

Goat polyclonal antibody to Calnexin

Research Area

Epigenetics, Infectious Diseases

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.1-1μg/ml, IHC-P: 5μg/ml, IF/ICC: 10μg/ml
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Mouse, Porcine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetCalnexin
Protein SequenceSKTPELNLDQFHDKT
Molecular Weight67.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti Calnexin antibody, anti CANX antibody, anti CNX antibody, anti IP90 antibody, anti IP 90 antibody, anti IP-90 antibody, anti Calnexin-CT antibody, anti Calnexin-NT antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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