Goat anti-carbonic anhydrase XII (aa188-199) Antibody

SKU: orb167716

Description

Goat polyclonal antibody to CA12.

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5-10µg/ml
Reactivity	Human
Application Notes	WB: Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1µg/ml.. IHC: In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	carbonic anhydrase XII (aa188-199)
Protein Sequence	SHLQHVKYKGQE
Molecular Weight	39.5; 38.4; 31.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti CA12 antibody, anti carbonic anhydrase XII antibody, anti CAXII antibody, anti HsT18816 antibody, anti CA-XII antibody, anti carbonate dehydratase XII antibody, anti carbonic anhydrase 12 antibody, anti carbonic dehydratase antibody, anti tumor antigen HOM-RCC-3.1.3 antibody

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Goat anti-carbonic anhydrase XII (aa188-199) Antibody

Western blot analysis of Human Kidney lysate (35ug protein in RIPA buffer) using CA12 antibody

Immunohistochemical staining of Human Kidney using CA12 antibody

Quick Database Links

Gene Symbol

carbonic anhydrase XII (aa188-199)

NCBI Gene ID

771

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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