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Goat Anti-CCR2 Antibody
SKU: orb2657253
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution range | ELISA: 1:16000. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6µg/ml. Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of THP-1 cells. Recommended concentration: 10µg/ml. |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Immunogen | Peptide with sequence RFIRNTNESGEEVT-C, from the N Terminus of the protein sequence according to NP_000638.1; NP_000639.1. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. |
| Disclaimer | For research use only |
Alternative Names
−chemokine (C-C motif) receptor 2, HGNC:1603, CC-CKR-2, CCR2A, CCR2B, CKR2, CKR2A, CKR2B, CMKBR2, MCP-1-R, MCP-1 receptor, chemokine (C-C) receptor 2, monocyte chemoattractant protein 1 receptor, monocyte chemotactic protein 1 receptor
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Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
6 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Quick Database Links
RefSeq (Protein):NP_000639.1, NP_000638.1
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000639.1, NP_000638.1 |
|---|
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Datasheet
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat Anti-CCR2 Antibody (orb2657253)
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