Goat anti-CD274 / PD-L1, Biotinylated Antibody

SKU: orb377881

Description

Goat polyclonal antibody against CD274 conjugated to Biotin.

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:128000, WB: 0.3-1 μg/ml
Reactivity	Human
Application Notes	WB: Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). Recommended concentration: 0.3-1μg/ml.

Key Properties

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Host	Goat
Target	CD274 / PD-L1, Biotinylated
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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CD274, CD274 molecule, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1

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Goat anti-CD274 / PD-L1, Biotinylated Antibody

Western blot analysis of human Heart lysate using CD274 antibody (Biotin)

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Gene Symbol

CD274 / PD-L1, Biotinylated

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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