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Goat anti-CD32 / FCGR2B Antibody
SKU: orb19010
Description
Images & Validation
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| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | CD32 / FCGR2B |
| Molecular Weight | 34.0; 31.9; 31.9; 34.0; 33.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti-CD32 antibody, anti- CD32 antigen antibody, anti- CD32B antibody, anti- CDw32 antibody, anti- Fc fragment of IgG, low affinity II, receptor for (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor antibody, anti- Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor for antibody, anti- Fc gamma receptor IIB antibody, anti- Fc gamma RII antibody, anti- Fc gamma RIIB antibody, anti- Fc-gamma RII antibody, anti- Fc-gamma RII-b antibody, anti- Fc-gamma-RIIb antibody, anti- FCG2 antibody, anti- FCG2B_human antibody, anti- Fcgr2 antibody, anti- FCGR2B antibody, anti- FCRII antibody, anti- FcRII-b antibody, anti- IGFR2 antibody, anti- IgG Fc receptor II beta antibody, anti- IgG Fc receptor II-b antibody, anti- Low affinity immunoglobulin gamma Fc region receptor II antibody, anti- Low affinity immunoglobulin gamma Fc region receptor II-b antibody, anti- Ly-17 antibody, anti- Lym 1 antibody, anti- Lymphocyte antigen 17 antibody
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1 μg/ml staining of Daudi cell lysate (RIPA buffer, 35 μg protein in RIPA buffer). Detected by chemiluminescence.
8 μg/ml staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
0.5 μg/ml staining of Human Placenta lysate (RIPA buffer, 35 μg protein in RIPA buffer). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-CD32 / FCGR2B Antibody (orb19010)
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