CFC1/Cripto-1 Antibody

SKU: orb19081

Description

Goat polyclonal antibody to CFC1

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC
Dilution range	ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1-2 μg/ml
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testes lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	CFC1 / Cripto-1
Molecular Weight	24.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti CFC1 antibody, anti HTX2 antibody, anti CRYPTIC antibody, anti cripto, FRL-1, cryptic family 1 antibody, anti cripto-1 antibody, anti cryptic antibody, anti cryptic gene antibody, anti MGC133213 antibody, anti CR-1 antibody, anti FLJ77897 antibody

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Immunohistochemical analysis of formalin-fixed and paraffin embedded Mouse Embryo Liver using CFC1 antibody

Quick Database Links

Gene Symbol

CFC1 / Cripto-1

NCBI Gene ID

55997

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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