Goat anti-COL4A6 Antibody

SKU: orb20338

Description

Goat polyclonal antibody to Collagen IV

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, FC, IF
Dilution Range	ELISA: 1:32000, IHC-P: 10ug/ml
Reactivity	Human
Application Notes	This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	COL4A6
Molecular Weight	164; 157; 161;158
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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COL4A6; collagen, type IV, alpha 6; MGC88184; OTTHUMP00000023834; OTTHUMP00000023835; collagen IV, alpha-6 polypeptide; collagen alpha 6 type IV; collagen of basement membrane, alpha-6; dJ889N15.4 (Collagen Alpha 6(IV)); type IV alpha 6 collagen

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Quick Database Links

Gene Symbol

COL4A6

NCBI Gene ID

1288

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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