Collagen, type VII Antibody

SKU: orb19514

Description

Goat polyclonal to Collagen, type VII

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 0.1-1.5 μg/ml
Reactivity	Rat
Predicted Reactivity	Canine, Human, Mouse, Porcine
Application Notes	This product does not work on cryostat sections of Normal Human Skin.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 250kDa band observed in rat testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5 μg/ml.Experiment Notes: A customer found this antibody unsuccessful on cryosections of Human Skin. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Collagen, type VII
Protein Sequence	TVQYSDDPRTEF
Molecular Weight	295
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Collagen, type VII antibody, anti collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive) antibody, anti COL7A1 antibody, anti HGNC:2214 antibody, anti EBD1 antibody, anti EBDCT antibody, anti EBR1 antibody, anti LC collagen antibody, anti alpha 1 type VII collagen antibody, anti collagen VII antibody, anti alpha-1 polypeptide antibody, anti long chain collagen antibody, anti collagen, type VII, alpha 1 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

orb19514 (0.5ug/ml) staining of rat testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Collagen, type VII

NCBI Gene ID

1294

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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