CYB5R3/Dia 1 (mouse) Antibody

SKU: orb20317

Description

Goat polyclonal antibody to Cyb5r3

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 0.1-1 μg/ml
Reactivity	Mouse
Predicted Reactivity	Bovine, Canine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kDa according to mouse NP_084063.1). Recommended concentration: 0.1-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	CYB5R3 / Dia 1 (mouse)
Protein Sequence	PNLERVGHPKERC
Molecular Weight	34.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Cyb5r3 antibody, anti cytochrome b5 reductase 3 antibody, anti 0610016L08Rik antibody, anti 2500002N19Rik antibody, anti C85115 antibody, anti Dia-1 antibody, anti Dia1 antibody, anti WU:AL591952.1-001 antibody, anti WU:AL591952.1-002 antibody, anti WU:AL591952.1-003 antibody, anti WU:Cyb5r3 antibody, anti NADH-cytochrome b5 reductase antibody, anti diaphorase 1 antibody, anti diaphorase 1 (NADH) antibody

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orb20317 (0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

CYB5R3 / Dia 1 (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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