EDD1/HYD Antibody

SKU: orb18490

Description

Goat polyclonal antibody to UBR5

Images & Validation

−

Item 1 of 2

Tested Applications	ELISA, IHC
Dilution range	ELISA: 1:4000, IHC-P: 1-2 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35-40kDa band observed in Human Testis, Human Skeletal Muscle, A549 and A431 lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 322kDa according to NP_056986 (furthermore, our SDS PAGE/western blotting protocol is not optimized for detection of proteins as large as the predicted). Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported Read more...

Key Properties

−

Host	Goat
Clonality	Polyclonal
Target	EDD1 / HYD
Molecular Weight	309
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

−

anti UBR5 antibody, anti EDD1 antibody, anti E3 ubiquitin protein ligase, HECT domain containing, 1 antibody, anti DD5 antibody, anti EDD antibody, anti HYD antibody, anti KIAA0896 antibody, anti progestin induced protein antibody, anti ubiquitin-protein ligase antibody, anti E3 identified by differential display antibody, anti hyperplastic discs protein homolog antibody, anti ubiquitin protein ligase E3 component n-recognin 5 antibody, anti FLJ11310 antibody, anti MGC57263 antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Immunohistochemical staining of Human Spleen using UBR5 antibody

Immunohistochemical staining of Human Testis using UBR5 antibody

Quick Database Links

Gene Symbol

EDD1 / HYD

NCBI Gene ID

51366

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols