Fgf14 (mouse N terminus) Antibody

SKU: orb12272

Description

Goat polyclonal antibody to Fgf14

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 1:5000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rat
Application Notes	WB: Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Fgf14 (mouse N terminus)
Molecular Weight	27.7; 28.3
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti Fgf14 antibody, anti FGF-14 antibody, anti Fhf4 antibody, anti FHF-4 antibody, anti fibroblast growth factor 14 antibody, anti fibroblast growth factor homologous factor 4 antibody, anti mFHF-4(1B) antibody

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Western blot analysis of Mouse Brain lysate using Fgf14 antibody

Quick Database Links

Gene Symbol

Fgf14 (mouse N terminus)

NCBI Gene ID

2259

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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