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GAD1 (isoform GAD67) Antibody

SKU: orb20081

Description

Goat polyclonal antibody to GAD1

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, FC, IF, WB
Dilution RangeELISA: 1:32000, WB: 0.5-1.5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetGAD1 (isoform GAD67)
Protein SequencePDSPQRREKLHK
Molecular Weight66.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GAD1 antibody, anti glutamate decarboxylase 1 (brain, 67kDa) antibody, anti FLJ45882 antibody, anti GAD antibody, anti SCP antibody, anti OTTHUMP00000041055 antibody, anti glutamate decarboxylase 1 antibody, anti glutamate decarboxylase 1 (brain, 67kD) antibody, anti GAD67 antibody, anti GAD25 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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