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GAPDH (C Terminus) Loading Control Antibody
SKU: orb18774
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml |
| Reactivity | Human, Rat |
| Predicted Reactivity | Canine, Mouse, Porcine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | GAPDH (C Terminus) Loading Control |
| Molecular Weight | 36.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody
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GAPDH (C Terminus) Loading Control, biotinylated Antibody [orb334083]
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0.1 µg/ml staining of Human Liver (A) and (1 ug/ml) Mouse Spleen (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
0.1 µg/ml staining of HEK293 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A549. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic, membrane and cytoplasmic bodies staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GAPDH (C Terminus) Loading Control Antibody (orb18774)
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