Goat anti-GEF5 Antibody

SKU: orb19152

Description

Goat polyclonal antibody to ARHGEF5

Research Area

Cancer, Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	GEF5
Protein Sequence	DKEIDQNSQQEE
Molecular Weight	177
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti GEF5 antibody, anti DKFZp686N1969 antibody, anti ARHGEF5 antibody, anti Rho guanine nucleotide exchange factor (GEF) 5 antibody, anti P60 antibody, anti TIM antibody, anti TIM1 antibody, anti guanine nucleotide regulatory protein TIM antibody, anti oncogene TIM rho guanine nucleotide exchange factor 5 antibody, anti transforming immortalized mammary oncogene antibody

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orb19152 (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Placenta using ARHGEF5 antibody

Quick Database Links

Gene Symbol

GEF5

NCBI Gene ID

7984

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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