GPR94/TRA1 Antibody

SKU: orb19279

Description

Goat polyclonal antibody to HSP90B1

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rat, Zebrafish
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx 100kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	GPR94 / TRA1
Molecular Weight	92.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti TRA1 antibody, anti GRP94 antibody, anti tumor rejection antigen (gp96) 1 antibody, anti HGNC:12028 antibody, anti ECGP antibody, anti GP96 antibody, anti Tumor rejection antigen-1 (gp96) antibody, anti endothelial cell (HBMEC) glycoprotein antibody, anti glucose regulated protein, 94 kDa antibody, anti tumor rejection antigen (gp96) 1 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

0.5 μg/ml staining of HeLa (A) and U2OS (B), and (0.3 μg/ml) on HepG2 (C), cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.

6 μg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.