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HMGI-C/HMGA2 Antibody

SKU: orb20345

Description

Goat polyclonal antibody to HMGA2

Research Area

Cancer, Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:8000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetHMGI-C / HMGA2
Protein SequenceKAAQKKAEATGEK
Molecular Weight11.8; 11.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti HMGA2 antibody, anti high mobility group AT-hook 2 antibody, anti BABL antibody, anti HMGI-C antibody, anti HMGIC antibody, anti LIPO antibody, anti STQTL9 antibody, anti High-mobility group protein HMGI-C antibody, anti high-mobility group (nonhistone chromosomal) protein isoform I-C antibody

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HMGI-C/HMGA2 Antibody

orb20345 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

HMGI-C/HMGA2 Antibody (orb20345)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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