HMGI-C/HMGA2 Antibody

SKU: orb1248600

Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Reactivity	Human
Predicted Reactivity	Canine
Application Notes	Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3µg/mL. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-KAAQKKAEATGEK
Target	HMGI-C / HMGA2
Molecular Weight	Approx 18kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HMGA2, high mobility group AT-hook 2, BABL, HMGI-C, HMGIC, LIPO, STQTL9, High-mobility group protein HMGI-C, high-mobility group (nonhistone chromosomal) protein isoform I-C

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orb1248600 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

HMGI-C / HMGA2

UniProt

P52926

RefSeq (Protein):NP_003474.1, NP_003475.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003474.1, NP_003475.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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