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Goat anti-HSPC150 / UBE2T Antibody
SKU: orb18482
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | HSPC150 / UBE2T |
| Molecular Weight | 22.5 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti UBE2T antibody, anti HSPC150 antibody, anti PIG50 antibody, anti HSPC150 protein similar to ubiquitin-conjugating enzyme antibody, anti ubiquitin conjugating enzyme antibody, anti ubiquitin-conjugating enzyme E2T (putative) antibody, anti ubiquitin-conjugating enzyme E2T antibody
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Primary incubation 1 hour at room temperature. Image A: K562 cell lysate at primary Ab concentration 0.1 µg/ml, Image B: THP-1 cell lysate at primary Ab concentration 0.3 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Primary incubation 1 hour at room temperature. Images A, B, C, D: A431, HEK293, HeLa, Jurkat nuclear cell lysate at primary Ab concentration 0.3 µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-HSPC150 / UBE2T Antibody (orb18482)
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