Goat anti-Ide (mouse) Antibody

SKU: orb19543

Description

Goat polyclonal antibody to Ide

Research Area

Infectious Diseases

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:16000, WB: 1-3 μg/ml
Reactivity	Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Ide (mouse)
Protein Sequence	QQYNYDRDNIE
Molecular Weight	118
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Ide antibody, anti insulin-degrading enzyme antibody, anti 1300012G03Rik antibody, anti 4833415K22Rik antibody, anti AA675336 antibody, anti AI507533 antibody, anti INSULYSIN antibody, anti insulinase antibody, anti insulysin antibody

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orb19543 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Ide (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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