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IDH3A Antibody
SKU: orb22494
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml |
| Reactivity | Human, Mouse, Porcine, Rat |
| Predicted Reactivity | Bovine, Canine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | IDH3A |
| Protein Sequence | DFTEEICRRVKDLD |
| Molecular Weight | 39.6 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti IDH3A antibody, anti isocitrate dehydrogenase 3 (NAD+) alpha antibody, anti H-IDH alpha antibody, anti NAD(+)-specific ICDH subunit alpha antibody, anti NAD(H)-specific isocitrate dehydrogenase alpha subunit antibody, anti NAD+-specific ICDH antibody, anti isocitrate dehydrogenase (NAD+) alpha chain antibody, anti isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial antibody, anti isocitric dehydrogenase subunit alpha antibody
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orb22494 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Spleen lysates using IDH3A antibody.
Western blot analysis of staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates using IDH3A antibody.
Western blot analysis of Human Lymph Nodes lysate (35ug protein in RIPA buffer) using IDH3A antibody
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IDH3A Antibody (orb22494)
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