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IGFBP6 Antibody

SKU: orb19859

Description

Goat polyclonal antibody to IGFBP6

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetIGFBP6
Molecular Weight25.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti IGFBP6 antibody, anti insulin-like growth factor binding protein 6 antibody, anti IBP6 antibody, anti IGF binding protein 6 antibody

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IGFBP6 Antibody

Western blot analysis of Human Adrenal Gland lysate using IGFBP6 antibody

IGFBP6 Antibody

Western blot analysis of HEK293 lysate using IGFBP6 antibody

IGFBP6 Antibody

orb19859 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IGFBP6 Antibody (orb19859)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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