Insulysin/Insulinase Antibody

SKU: orb19588

Description

Goat polyclonal antibody to IDE

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
Reactivity	Human
Predicted Reactivity	Canine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Insulysin / Insulinase
Molecular Weight	118, 54
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti insulinase antibody, anti insulysin antibody, anti insulin-degrading enzyme antibody, anti FLJ35968 antibody, anti INSULYSIN antibody, anti IDE antibody

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Western blot analysis of K562 cell lysate using IDE antibody

Western blot analysis of HEK293 lysate using IDE antibody

Immunohistochemical staining of Human Lung using IDE antibody

Immunohistochemical staining of Human Breast using IDE antibody

Quick Database Links

Gene Symbol

Insulysin / Insulinase

NCBI Gene ID

3416

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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