Klk6/neurosin (mouse) Antibody

SKU: orb20308

Description

Goat polyclonal antibody to Klk6

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:16000, WB: 1-3 μg/ml
Reactivity	Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 22kDa band observed in Mouse Liver lysates (calculated MW of 27.5kDa according to NP_035307.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. Both bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Klk6 / neurosin (mouse)
Molecular Weight	27.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti Klk6 antibody, anti kallikrein related-peptidase 6 antibody, anti AI849898 antibody, anti Bssp antibody, anti Klk29 antibody, anti Klk7 antibody, anti Prss18 antibody, anti Prss9 antibody, anti neurosin antibody, anti kallikrein 29 antibody, anti kallikrein 6 antibody, anti kallikrein 7 antibody, anti protease M antibody, anti protease, serine, 18 antibody, anti protease, serine, 9 antibody

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orb20308 staining (2ug/ml) of Mouse Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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Gene Symbol

Klk6 / neurosin (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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