KPNA3/IPOA4 Antibody

SKU: orb18610

Description

Goat polyclonal antibody to KPNA3

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.1-0.3 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Heart shows strong pixulate staining of myocardial fibres in transverse section. Recommended concentration, 5-10ug/ml.WB: Approx 55-60kDa band observed in Human Testis and Kidney lysates (predicted size of approx. 58kDa according to NP_002258). Recommended for use at 0.1-0.5 μg/ml Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	KPNA3 / IPOA4
Protein Sequence	DPTANLQTKEFNF
Molecular Weight	57.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti KPNA3 antibody, anti IPOA4 antibody, anti karyopherin alpha 3 (importin alpha 4) antibody, anti RP11-432M24.3 antibody, anti SRP1gamma antibody, anti SRP4 antibody, anti hSRP1 antibody, anti importin alpha 4 antibody, anti importin alpha 4 antibody, anti importin-alpha-Q2 antibody, anti karyopherin alpha 3 antibody, anti SRP1 antibody, anti importin alpha-3 antibody

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orb18610 staining (0.1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18610 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of HeLa lysate using KPNA3 antibody

Immunohistochemical staining of Human heart using KPNA3 antibody

Quick Database Links

Gene Symbol

KPNA3 / IPOA4

NCBI Gene ID

3839

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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