NCF1/p47phox Antibody

SKU: orb18301

This product can be custom made

Description

Goat polyclonal antibody to NCF1

Research Area

Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA: 1:20000, WB: 0.01-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human, Mouse, Porcine
Predicted Reactivity	Bovine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 45kDa band observed in lysates of cell line U937 (calculated MW of 44.7kDa according to NP_000256.3). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	NCF1 / p47phox
Protein Sequence	SESTKRKLASAV
Molecular Weight	44.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti NCF1 antibody, anti p47phox antibody, anti FLJ79451 antibody, anti NCF1A antibody, anti NOXO2 antibody, anti SH3PXD1A antibody, anti neutrophil cytosolic factor 1 antibody, anti Neutrophil cytosolic factor-1 (47kD) antibody, anti neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) antibody, anti NADPH oxidase organizer 2 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of U937 cell lysate using NCF1 antibody

Western blot analysis of Mouse Thymus (Lane 1) and Pig Spleen (Lane 2) lysate using NCF1 antibody

Immunohistochemical staining of Human Colon using NCF1 antibody

Quick Database Links

Gene Symbol

NCF1 / p47phox

NCBI Gene ID

653361

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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