NIR1/PITPNM3 Antibody

SKU: orb18873

Description

Goat polyclonal antibody to PITPNM3

Research Area

Epigenetics, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC
Dilution Range	ELISA: 1:16000, WB: 0.1 μg/ml, IHC-P: 5-10µg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1 μg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	NIR1 / PITPNM3
Protein Sequence	SWARGPPKFESVP
Molecular Weight	107; 103
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti NIR1 antibody, anti PITPNM family member 3 antibody, anti PYK2 N-terminal domain-interacting receptor 1 antibody, anti RDGBA3 antibody, anti PITPNM3 antibody, anti CORD5 antibody, anti MGC157740 antibody, anti MGC157741 antibody, anti membrane-associated phosphatidylinositol transfer protein 3 antibody, anti retinal degeneration B alpha 3 antibody

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orb18873 (5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

NIR1 / PITPNM3

NCBI Gene ID

83394

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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