NPHS2/SRN1 Antibody

SKU: orb131711

Description

Goat polyclonal to NPHS2

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:64000, WB: 0.5-2 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Porcine
Application Notes	Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.WB: Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	NPHS2 / SRN1
Molecular Weight	42.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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NPHS2; nephrosis 2, idiopathic, steroid-resistant (podocin); PDCN; SRN1; podocin

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Western blot analysis of taining of Human (A) and Mouse (B) Kidney lysates using NPHS2 antibody.

Western blot analysis of staining of Pig Kidney lysates using NPHS2 antibody.

Western blot analysis of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer) using NPHS2 antibody

Quick Database Links

Gene Symbol

NPHS2 / SRN1

NCBI Gene ID

7827

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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