NPHS2/SRN1 Antibody

SKU: orb1248379

Description

NPHS2/SRN1 Antibody

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Porcine
Application Notes	Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to Human NP_055440.1 and Mouse NP_569723.1). Recommended concentration: 0.5-2µg/mL. Primary incubation 1 hour at room temperature.

Key Properties

−

Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-SPSKPVEPLNPKK
Target	NPHS2 / SRN1
Molecular Weight	Approx 45kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Disclaimer	For research use only

Alternative Names

−

NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), PDCN, SRN1, podocin

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

orb1248379 (1 ug/ml) staining of Human (A) and Mouse (B) Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NPHS2 / SRN1

UniProt

Q9NP85

RefSeq (Protein):NP_055440.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_055440.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols