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PCBP4 Antibody
SKU: orb19596
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:128000, WB: 1-3 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | PCBP4 |
| Protein Sequence | NGSKKAERQKFSPY |
| Molecular Weight | 43.9, 37.9, 41.5, 41.5 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti PCBP4 antibody, anti poly(rC) binding protein 4 antibody, anti LIP4 antibody, anti MCG10 antibody, anti LYST-interacting protein antibody, anti RNA binding protein MCG10 antibody, anti alphaCP-4 protein antibody, anti poly(rC)-binding protein 4 antibody, anti alpha CP-4 antibody
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orb19596 (0.03ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing PCBP4 (RC200749) and probed with orb19596 (mock transfection in first lane), tested by Origene.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PCBP4 Antibody (orb19596)
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