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Pirin Antibody

SKU: orb18865

Description

Goat polyclonal antibody to PIR

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.3 - 1.0 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetPirin
Protein SequenceGSSKKVTLSVLSREC
Molecular Weight32.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti pirin antibody, anti PIR antibody, anti pirin (iron-binding nuclear protein) antibody

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Pirin Antibody

Western blot analysis of Human Heart lysate using PIR antibody

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Western blot analysis of HEK293 lysate using PIR antibody

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Immunohistochemical staining of Human Liver using PIR antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Pirin Antibody (orb18865)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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