Goat anti-POLL / POLkappa Antibody

SKU: orb18585

Description

Goat polyclonal antibody to POLL

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 0.5-1 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	POLL / POLkappa
Protein Sequence	LGLPYREPAERDW
Molecular Weight	63.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti POLL antibody, anti BETA-N antibody, anti POL-KAPPA antibody, anti polymerase (DNA directed), lambda antibody, anti polymerase (DNA-directed), lambda antibody, anti DNA polymerase kappa (DNA polymerase beta-N) antibody, anti RP11-529I10.1 antibody, anti BETAN antibody, anti FLJ46002 antibody, anti POLKAPPA antibody, anti DNA polymerase beta-2 antibody, anti DNA polymerase kappa (DNA polymerase beta-N) antibody, anti DNA polymerase lamda2 antibody, anti DNA-directed DNA polymerase lambda antibody, anti OTTHUMP00000020323 antibody, anti pol beta2 antibody, anti pol lambda antibody

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Immunohistochemical staining of Human Small Intestine using POLL antibody

Western blot analysis of A549 (Lane 1) and HepG2 (Lane 2) cell lysate using POLL antibody

Quick Database Links

Gene Symbol

POLL / POLkappa

NCBI Gene ID

27343

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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