PTBP1/PTB Antibody

SKU: orb18567

Description

Goat polyclonal antibody to PTBP1

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:32000, WB: 0.01-0.3 μg/ml, IHC-P: 2-4 μg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 5-10ug/ml.WB: Approx 55-60 kDa band observed in lysates of cell line Jurkat (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PTBP1 / PTB
Protein Sequence	DGIVPDIAVGTKR
Molecular Weight	59.6, 59.0, 57.2; 21.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti PTBP1 antibody, anti PTB antibody, anti polypyrimidine tract binding protein 1 antibody, anti PTB2 antibody, anti PTB3 antibody, anti PTB4 antibody, anti pPTB antibody, anti HNRPI antibody, anti PTB-1 antibody, anti HNRNPI antibody, anti RNA binding protein antibody, anti heterogeneous nuclear ribonucleoprotein polypeptide I antibody, anti polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody, anti HNRNP-I antibody, anti MGC10830 antibody, anti MGC8461 antibody, anti RNA-binding protein antibody, anti heterogeneous nuclear ribonucleoprotein I antibody

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Western blot analysis of staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer) using PTBP1 antibody.

Western blot analysis of staining of Human Ovary lysate (35µg protein in RIPA buffer) using PTBP1 antibody.

Immunohistochemical analysis of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining with PTBP1 antibody.

Western blot analysis of lysates of cell line NIH3T3 using PTBP1 antibody

Quick Database Links

Gene Symbol

PTBP1 / PTB

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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