S100A4/CAPL Antibody

SKU: orb19362

Featured

Description

Goat polyclonal antibody to S100A4

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

−

Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 1-2 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Small Intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells . Recommended concentration, 2-3ug/ml.WB: Approx 13kDa band observed in Human Placenta lysates (calculated MW of 11.7kDa according to NP_002952.1and NP_062427.1). Recommended concentration: 0.03-0.1 μg/ml. Read more...

Key Properties

−

Host	Goat
Clonality	Polyclonal
Target	S100A4 / CAPL
Protein Sequence	CNEFFEGFPDKQP
Molecular Weight	11.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

anti S100A4 antibody, anti CAPL antibody, anti S100 calcium binding protein A4 antibody, anti 18A2 antibody, anti 42A antibody, anti MTS1 antibody, anti P9KA antibody, anti PEL98 antibody, anti S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody, anti S100 calcium-binding protein A4 antibody, anti S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody, anti malignant transformation suppression 1 antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of Human Placenta lysate using S100A4 antibody

Immunohistochemical of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Small Intestine using S100A4 antibody

Quick Database Links

Gene Symbol

S100A4 / CAPL

NCBI Gene ID

6275

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Filter by Applications

IF (1)

Filter by Species

Human (1)

Vogel, Sebastian et al. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET J Biol Chem, 289, 11068-11082 (2014)

PubMed: 24567328

Applications

Reactivity

Human