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SEPT7 Antibody

SKU: orb20054

Description

Goat polyclonal antibody to 41889

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:32000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 45kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7kDa according to NP_001779.3 and of 55.6kDa according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetSEPT7
Protein SequenceDNNKNKGQLTKSP
Molecular Weight50.7; 50.6; 46.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti SEPT7 antibody, anti septin 7 antibody, anti CDC10 antibody, anti CDC3 antibody, anti Nbla02942 antibody, anti SEPT7A antibody, anti CDC10 (cell division cycle 10, S. cerevisiae, homolog) antibody, anti CDC10 cell division cycle 10 homolog antibody, anti cell division cycle 10 antibody

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SEPT7 Antibody

orb20054 (0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SEPT7 Antibody (orb20054)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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