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SIRT4 Antibody
SKU: orb18632
Description
Research Area
Cancer, Cardiovascular Disease, DNA Damage
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-3 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | SIRT4 |
| Protein Sequence | SRCGELLPLIDPC |
| Molecular Weight | 35.2 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti SIRT4 antibody, anti SIR2L4 antibody, anti sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody, anti sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae) antibody, anti MGC130046 antibody, anti MGC130047 antibody, anti MGC57437 antibody, anti SIR2L4 antibody, anti sirtuin 4 antibody, anti sir2-like 4 antibody, anti sirtuin 4 antibody, anti sirtuin type 4 antibody
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orb18632 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18632 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SIRT4 Antibody (orb18632)
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