SLC2A4/GLUT4 Antibody

SKU: orb20638

Description

Goat polyclonal to GLUT4

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	SLC2A4 / GLUT4
Molecular Weight	54.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti SLC2A4 antibody, anti solute carrier family 2 (facilitated glucose transporter), member 4 antibody, anti GLUT4 antibody, anti glucose transporter 4 antibody, anti insulin-responsive glucose transporter type 4 antibody

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Western blot analysis of Mouse Heart lysate using GLUT4 antibody

Quick Database Links

Gene Symbol

SLC2A4 / GLUT4

NCBI Gene ID

6517

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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