Goat anti-SPA1 / SIPA1 Antibody

SKU: orb18646

Description

Goat polyclonal antibody to SIPA1

Research Area

Epigenetics, Stem Cells

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 1-3 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3 μg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	SPA1 / SIPA1
Protein Sequence	ASKQLGSPTADLA
Molecular Weight	112
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti SIPA1 antibody, anti SPA1 antibody, anti MGC17037 antibody, anti signal-induced proliferation-associated gene 1 antibody, anti p130 SPA-1 antibody, anti GTPase-activating protein Spa-1 antibody, anti signal-induced proliferation-associated 1 antibody, anti MGC102688 antibody, anti signal-induced proliferation-associated gene 1 antibody, anti signal-induced proliferation-associated protein 1 antibody

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orb18646 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

SPA1 / SIPA1

NCBI Gene ID

6494

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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