Goat anti-TCP1 Antibody

SKU: orb18647

Description

Goat polyclonal antibody to TCP1

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 1-2 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration, 1-2ug/ml.WB: Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	TCP1
Molecular Weight	60.3; 43.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti TCP1 antibody, anti t-complex 1 antibody, anti CCT-alpha antibody, anti CCT1 antibody, anti CCTa antibody, anti D6S230E antibody, anti TCP-1-alpha antibody, anti T-complex protein 1 antibody, anti T-complex protein 1, alpha subunit antibody, anti tailless complex polypeptide 1 antibody

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Immunohistochemical staining of Human Testis using TCP1 antibody

Western blot analysis of Human Ovary (lane 1) lysate and HEK293 (lane 2) cell lysate using TCP1 antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using TCP1 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using TCP1 antibody.

Quick Database Links

Gene Symbol

TCP1

NCBI Gene ID

6950

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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