Goat anti-Themis (mouse) Antibody

SKU: orb20519

Description

Goat polyclonal to Themis

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:64000, WB: 1-3 μg/ml
Reactivity	Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 72kDa band observed in Mouse Spleen lysates (calculated MW of 72.7kDa according to NP_848781.1). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Themis (mouse)
Molecular Weight	72.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti THEMIS antibody, anti thymocyte selection associated antibody, anti C6orf190 antibody, anti C6orf207 antibody, anti FLJ40584 antibody, anti MGC163388 antibody, anti TSEPA antibody, anti bA325O24.3 antibody, anti bA325O24.4 antibody, anti thymocyte selection pathway associated antibody, anti thymocyte-expressed molecule involved in selection antibody

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orb20519 (0.3ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Themis (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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