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TRPM7/LTRPC7 Antibody
SKU: orb18297
Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution range | ELISA: 1:8000, IHC-P: 4-6 μg/ml |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Canine, Porcine, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | TRPM7 / LTRPC7 |
| Molecular Weight | 213; 213 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti TRPM7 antibody, anti transient receptor potential cation channel, subfamily M, member 7 antibody, anti CHAK antibody, anti CHAK1 antibody, anti LTRPC7 antibody, anti FLJ20117 antibody, anti TRP-PLIK antibody, anti LTRPC ion channel family member 7 antibody, anti homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein FLJ20117 antibody, anti FLJ25718 antibody
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Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
6 µg/ml staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TRPM7/LTRPC7 Antibody (orb18297)
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