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Goat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb347002

Description

Goat IgG F(ab')2 antibody (Alkaline Phosphatase)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
ReactivityGoat
Application Notes
This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 is suggested for this product.

Key Properties

Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenGoat IgG F(ab')2 fragment
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum. No reaction was observed against Goat IgG F(c) or Human Serum Proteins.
ConjugationAP

Storage & Handling

StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
DisclaimerFor research use only

Alternative Names

rabbit anti-goat IgG F(ab')2 ALP conjugated, rabbit anti-goat IgG F(ab')2 alkaline phosphatase conjugated, rabbit anti-goat IgG Fab2 ALP conjugated, rabbit anti-goat IgG Fab2 alkaline phosphatase conjugated

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Goat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed (orb347002)

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500 μg
$ 520.00
DispatchUsually dispatched within 5-10 working days
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